Affinity Chromatography - download pdf or read online

By Jaroslava Turková (Eds.)

ISBN-10: 0444416056

ISBN-13: 9780444416056

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J. , Eur. J. Biochem.. 42 (1974b) 1-6. H. ,Biopolymers, 1 (1963) 133-143. , Bailon, P. , Advan. Exp. Med. , 42 (1974) 33-42. , Tanabe, S. , Biopolymers, 14 (1975) 33-49. , 34 (1974) 13-30. Porath, J. , in H. L. 95-178. H. , Chromarographia, 4 (1971) 578-581. , Cuatrecasas, P. , J. Bwl. , 246 (1971) 196-200. , Coupek, J. ,Eiochim. Biophys, Acta, 427 (1976) 586-593. 35 Chapter 4 Application of affinity chromatography to the quantitative evaluation of specific complexes Association processes undoubtedly play one of the most important roles in biochemical processes.

In Fig. 3%of the enzyme being lost during the washing steps. 7% of the enzyme can be recovered. 2% and for K i = M it is only 16%. Fig. 7C illustrates a very favourable case for K L = lo-’ M or less. In this instance virtually no enzyme is lost during the washing. 9% of the amount of the starting enzyme after 20 washes. High K L values are desirable, because they lead to much more concentrated solutions of the recovered enzyme. a 11 =OM TOTAL WASHES A N D ELUTIONS. cIv'+Iw)tv Fig. 7. Simulated column results for (A) a poor binding case (KL = lo-' M) where elution is achieved by a change in KL t o KL; considerable enzyme loss occurs during washing; (B) moderately good binding (KL = lo-' M); little enzyme is lost during washing; (C) very good binding (KL < practically no enzyme is lost by washing.

Eur. J. Biochem.. 42 (1974b) 1-6. H. ,Biopolymers, 1 (1963) 133-143. , Bailon, P. , Advan. Exp. Med. , 42 (1974) 33-42. , Tanabe, S. , Biopolymers, 14 (1975) 33-49. , 34 (1974) 13-30. Porath, J. , in H. L. 95-178. H. , Chromarographia, 4 (1971) 578-581. , Cuatrecasas, P. , J. Bwl. , 246 (1971) 196-200. , Coupek, J. ,Eiochim. Biophys, Acta, 427 (1976) 586-593. 35 Chapter 4 Application of affinity chromatography to the quantitative evaluation of specific complexes Association processes undoubtedly play one of the most important roles in biochemical processes.

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Affinity Chromatography by Jaroslava Turková (Eds.)


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